Phage Display Libraries for Antibody Therapeutic Discovery and Development
نویسندگان
چکیده
منابع مشابه
The role of phage display in therapeutic antibody discovery.
Phage display involves the expression of selected proteins on the surface of filamentous phage through fusion with phage coat protein, with the genetic sequence packaged within, linking phenotype to genotype selection. When combined with antibody libraries, phage display allows for rapid in vitro selection of antigen-specific antibodies and recovery of their corresponding coding sequence. Large...
متن کاملPhage display of combinatorial antibody libraries.
The selection of antibodies from combinatorial libraries displayed on the surface of filamentous phage has become an important methodology for the generation of reagent, diagnostic, and therapeutic molecules and for the study of natural immune responses. Using this technique, antibody genes have been cloned from multiple species or expressed directly from large man-made repertoires of antibody-...
متن کاملAntibody Phage Display Libraries: Contributions to Oncology
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside can...
متن کاملTargeting membrane proteins for antibody discovery using phage display
A critical factor in the successful isolation of new antibodies by phage display is the presentation of a correctly folded antigen. While this is relatively simple for soluble proteins which can be purified and immobilized onto a plastic surface, membrane proteins offer significant challenges for antibody discovery. Whole cell panning allows presentation of the membrane protein in its native co...
متن کاملProbing antinuclear antibody specificities by peptide phage display libraries.
OBJECTIVE To uncover the specificities of autoantibodies to nuclear proteins (ANA) in patients with juvenile rheumatoid arthritis (JRA). METHODS Peptide ligands for ANA were selected by panning random peptide phage display libraries on antibodies binding to HEp-2 cells. Positive phage clones were identified by the immunoscreening technique. RESULTS Groups of peptides were identified, some o...
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ژورنال
عنوان ژورنال: Antibodies
سال: 2019
ISSN: 2073-4468
DOI: 10.3390/antib8030044